It ensures higher sensitivity, longer pcr products and higher yields compared to conventional taq dna polymerase. Ab0192kcl 1 thermo scientific taq dna polymerase with kcl buffer description. Taq dna polymerase definition of taq dna polymerase by. Due to these features pcr protocols with phire hot start ii dna polymerase can be significantly faster than protocols with taq dna polymerases. Product information thermo scientific dreamtaq dna. Taq dna polymerase is the original and most commonly used pcr enzyme. Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1.
Platinum taq dna polymerase from thermo fisher scientific. Phusion highfidelity dna polymerase processivity that is 10fold greater than pyrococcus furiosus dna polymerase and twice that of taq dna polymerase. Commercial producers have sinced cloned and expressed the enzyme in e. Thermo scientific taq dna polymerase, recombinant 5 ul. The enzyme catalyzes the templatedependent polymerization of nucleotides into duplex dna in the 5 3 direction. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus, from which it was originally isolated by chien et al. The gene that encoded the taq dna polymerase had been cloned and expressed in escherichia coli efficiently. Pcr protocol for taq dna polymerase with thermopol buffer m0267.
The taq pcr master mix kit includes qiagens taq dna polymerase in a premixed format. Onehalf of the total reaction volume is usually sufficient. The optimal amount of taq polymerase to be used per reaction varies with the amplicon length. Pcr protocol for taq dna polymerase with thermopol buffer. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. Thermo scientific dreamtaq dna polymerase is an enhanced taq dna polymerase optimized for all standard pcr. Optimize routine pcr with this highly thermostable dna polymerase. It can also be used for longrange pcr up to 20 kb from genomic dna templates and up to 30 kb from lambda dna templates. Thermo scientific taq dna polymerase, recombinant 1 ul 1. Thermo scientific dreamtaq dna polymerase is an enhanced taq dna polymerase optimized for all standard pcr applications. Separate components pcr reaction setup use the measurements below to prepare your pcr experiment, or enter your own parameters in the column provided.
Pcr protocol for taq dna polymerase with standard taq buffer m0273. Platinum taq dna polymerase thermo fisher scientific. Taq dna polymerase is a highquality recombinant enzyme that is suitable for general and specialized pcr applications see figures tolerance of different primer t m values and specific amplification of long pcr products qiagen pcr buffer. Add all components as in the basic pcr protocol, except for the taq dna polymerase. Platinum taq dna polymerase is a convenient and reliable hot start thermostable dna polymerase for pcr that provides enhanced specificity over that of taq dna polymerase. Platinum ii taq hotstart dna polymerase technology networks. Promega manufacturing and delivery systems continue to be fully operational during the covid19. The polymerase chain reaction pcr is an important technique for biology students to learn. Cycling conditions amplification parameters depend greatly on the template, primers and amplification apparatus. Taq dna polymerase, recombinant thermo fisher scientific. Taq dna polymerase pcr buffer thermo fisher scientific.
Consider the following guidelines when designing pcr strategies using taq dna polymerase. Taq dna polymerase is typically stored in a 50% glycerol solution and for complete dispersal in the reaction mix requires gentle mixing of the pcr reagents by pipetting up and down at least 20 times. The enzyme has terminal transferase activity which results in the addition of a single nucleotide adenosine at 3 end of the extension product. It is supplied with 10x thermopol reaction buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations. A simple and efficient method for extraction of taq dna. The enzyme catalyzes 53 synthesis of dna, has no detectable 35 exonuclease proofreading activity and possesses low 53 exonuclease activity. First, the recombinant strain biomass was obtained by inoculation of autoinduction medium with bacterial colonies from agar plate. Product information pfu dna polymerase, recombinant. Pluthero 1993 rapid purification of highactivity taq dna polymerase.
Ex taq polymerase has a higher fidelity than standard taq with a. The enzyme catalyzes 5 3 synthesis of dna, has no detectable 3 5 exonuclease proofreading activity and possesses low 5 3 exonuclease activity. This product is manufactured by new england biolabs, inc. Polymerase provides cycling speed, universal cycling protocol and market leading.
Dreamtaq dna polymerase product information igem 2016. The processivity of phusion dna polymerases is approximately 10fold greater than that of pfu dna polymerase and. Taq dna polymerase is a thermostable dna polymerase isolated from an e. Routine, hot start, highfidelity and direct pcr enzymes. Like our standard dreamtaq dna polymerase, this hotstart polymerase offers higher yields and longer amplicons than conventional taqbased products. Day 1 start 3ml overnight culture of taq from glycerol stock in lbamp 75ngul day 2 add 1ml of overnight culture to 1l of lbamp. Dreamtaq hot start dna polymerase is the hotstart version of our enhanced thermo scientific dreamtaq dna polymerase. This product is supplied with 10x reaction buffer containing 15 mm magnesium chloride. Takara taq hs polymerase is an antibodymediated hotstart version of takara taq dna polymerase, which is a recombinant version of fulllength taq.
Benefits of invitrogen platinum ii taq hotstart dna polymerase. Pcr is a most widelyused technique in biology, which utilizes thermostable dna polymerase from thermus aquaticus taq. Thermo scientific taq dna polymerase is a highly thermostable dna polymerase from the thermophilic bacterium thermus aquaticus. Weve detected that you are using an older version of internet explorer. The enzyme consists of a single polypeptide with a molecular weight of 94 kda. To prepare several parallel reactions and to minimize the possibility of pipetting errors, prepare a pcr. Purification of thermostable dna polymerases gene and. Determination of taq polymerase units using an authentic taq polymerase standard. The enzyme is a taq dna polymerase with buffers designed for enhanced amplification. Compare taq polymerase from thermo fisher scientific from leading suppliers on biocompare. This has greatly facilitated the production and reduced the price of the enzyme. Taq dna polymerase, recombinant 5 ul thermo fisher scientific.
Thermo scientific pfu dna polymerase is a highly thermostable dna polymerase from the hyperthermophilic archaeum pyrococcus furiosus. The taq dna polymerase was isolated as described in with some modifications making the purification protocol simpler and more convenient. Due to the difficulties in pipetting small volumes of enzyme, taq dna polymerase can be diluted in 1x reaction buffer. It is frequently used in the polymerase chain reaction pcr, a method for greatly amplifying the quantity of short segments of dna. Thermo scientific products include well known brands such as phusion and dreamtaq dna polymerase. Invitrogen taq dna polymerase, recombinant 500 units. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr. Thermo scientific taq dna polymerase is a highly thermostable dna polymerase. Taq dna polymerase is appropriate for use in the amplification of dna from complex genomic, viral, and plasmid templates, rtpcr, sequencing ssdna, and cycle sequencing unit definition. Pcr utilizes dna polymerases isolated from archaea or bacteria, like thermus aquaticus taq, to. Only primers and template dna need to be added to set up pcr. A convenient 2x pcr master mix which consist of takara taq hs polymerase, optimized reaction buffer, and dntps. Dreamtaq hot start dna polymerase thermo fisher scientific. Dreamtaq dna polymerase uses the same reaction setup and cycling conditions as conventional taq dna polymerase.
The micropipettor should be set to about half the reaction volume of the master mix when mixing, and care should be taken to avoid introducing. The recombinant protein will be purified from an e. First isolated from the bacterial thermophile thermus aquaticus, the taq polymerase has become the staple enzyme in molecular biology. Phire hot start ii dna polymerase thermo fisher scientific us. Innovative qiagen pcr buffer has been developed to save time and effort by reducing the need for pcr optimization. Taq polymerase from thermo fisher scientific biocompare.